Протеинова експресия се отнася до синтеза на протеини within cells using information contained in the DNA or gene.
Протеини are responsible for all the biochemical reactions that take place within the cell. Hence, it makes it necessary to study протеин function in order to understand cellular processes.
This is currently studied based on using fluorescent протеини as tags. However, this does not provide real time analysis as it requires maturation of chromophore that takes time and this causes a delay in investigation of real-time expression, especially for протеини които са преходни или ефимерни по природа.
Изследователите съобщиха за нова техника на сървъра за предпечат на 30 юли 2020 г., която може да преодолее това ограничение.
The new study describes the use a fluorescent biosensor that enables real-time detection of протеин expression in vivo which has implications for understanding the spatiotemporal expression of протеини within the living organism. This sensor is based on a dim green fluorescent протеин in which pre-existing fluorescence increases 11-fold in vivo following the specific and rapid binding of a протеин tag and enables detection of протеин expression within seconds in live cells.
Това биосензор will be useful to study biological processes in real-time where протеини are either transiently expressed and/or to understand the expression and interactions of протеини from a disease-causing organism such as bacteria or a virus with that of the host proteins.
справка:
Eason MG., Pandelieva AT., Mayer MM., et al 2020. Генетично кодиран флуоресцентен биосензор за бързо откриване на протеинова експресия. Предпечат: bioRxiv 2020.07.30.229633; DOI: https://doi.org/10.1101/2020.07.30.229633
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